Molecular Medicine and Diagnostics

Biography

Biography: Vladislav S Baranov

Abstract

Endometriosis (E) is a common multigenetic disorder affecting almost 10% of women of reproductive age. Complex molecular, genetic, immunological analysis and endocrinology tests were applied in the studies of 257 women with E and in 117 women in the control Participation of the genes responsible for steroid hormone activity, their receptors, inflammation, proliferation, cell migration, apoptosis, intercellular adhesion, angiogenesis as well as the genes regulating their activity have been suggested as plausible candidates. A handful of very interesting new candidate genes involved in oncogenesis , metaplasia of endometrium cells and embryonic development of female reproductive system were identified by GWAS technology and also by conventional genetic testing. Genetic polymorphisms and differential expression of the candidate genes regulated by different epigenetic factors were thoroughly studied and substantiated complex epigenetic landscape of E . According to recent hypothesis ( Baranov et al,2015) E could be induced by different combinations of relevant genetic and epigenetic factors with subsequent canalization of pathological process , which soon becomes irreversible and inevitably proceeds to clinical manifestations following “ direct or reverse epigenetic landscapes” routs. Two hypothetical stages of E include: the origin of primary incipient endometrioitic cells (PIEC) resulting as transition products of emdometrial epithelia to mesenchymal cells ( metaplasia), as well as from vestigial embryonic or dormant stem cells (St 1) ; progression of PIEC into E. lesions augmented by numerous genetic and epigenetic factors ( St.2) . Identification PIEC cells and complex molecular genetic analysis of their origin and progression may be fruitful in diagnostic biomarker search and may substantiate further advancement of prediction, prevention and treatment of E .