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 Confidence Oyebuchi Ukaeje

Confidence Oyebuchi Ukaeje

Cyprus International University, Cyprus

Title: Physiochemical characterization, antioxidative, cancer cells toxicity and food pathogen antibacterial activity of chitosan nanoparticles loaded with Cyperus articulatus essential oils


Biography: Confidence Oyebuchi Ukaeje


Essential oil of Cyperus articulatus has recognized fungicidal and antibacterial properties the qualitative chemical composition analysis by Gas Chromatography-Mass Spectrometry (GC-MS) revealed the presence of sesquiterpenes, monoterpenes, hydrocarbons and other esters, nootkatone, 6-methyl-3,5-heptadien-2- one, retinine, nopinone, cycloeucalenol, anozol, toosendanin, furanone, ethanone and vitamin-A. Cyperus articulatus Essential Oils (CPEOs) loaded Chitosan Nanoparticles (CSNPs) was successful synthesized using an oil-in-water emulsion and ionic gelation method. The samples were characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and UV-vis spectrophotometry. CPEOs had a higher radical-scavenging activity than CSNPs and CPEO-CSNPs at initial storage times. CPEO-CSNPs showed the highest (P<0.05) antioxidant activity over prolonged period of time. Antimicrobial activity of five strains of bacteria, Staphylococcus aureus (ATCC6538), Salmonella typhimurium (ATCC 14028), Listeria monocytogenes (ATCC 19112), Bacillus cereus (LMG 22730), and Escherichia coli (ATCC 8739) showed that all CPEO loaded chitosan nanoparticle inhibited bacterial growth at lower (P<0.05) CPEO loaded chitosan nanoparticle (5 mg/mL MIC) compared to free CPEO (10 mg/mL MIC).